Which influenza vaccine types are not prepared using embryonated egg culture?

Which influenza vaccine types are not prepared using embryonated egg culture?

• A. Live attenuated intranasal vaccine
• B. All influenza vaccines are egg-based
• C. Egg-based vaccines
• D. Recombinant and cell-culture-based inactivated vaccines

Answer: (D)

Many influenza vaccines are made by growing the virus in embryonated chicken eggs, which is the traditional egg-based production method. However, some vaccines avoid eggs entirely. Recombinant influenza vaccines contain the hemagglutinin protein produced by recombinant DNA technology in insect cell culture, so no eggs are involved. Cell-culture-based inactivated vaccines grow the virus in mammalian cell lines (such as MDCK cells) instead of eggs and then inactivate the virus. Because these methods do not use embryonated eggs, they are not prepared with egg culture. The intranasal live attenuated vaccine, while often produced using egg-based methods historically, is not the non-egg group and isn’t the best example of a non-egg-produced option.

Many influenza vaccines are made by growing the virus in embryonated chicken eggs, which is the traditional egg-based production method. However, some vaccines avoid eggs entirely. Recombinant influenza vaccines contain the hemagglutinin protein produced by recombinant DNA technology in insect cell culture, so no eggs are involved. Cell-culture-based inactivated vaccines grow the virus in mammalian cell lines (such as MDCK cells) instead of eggs and then inactivate the virus. Because these methods do not use embryonated eggs, they are not prepared with egg culture. The intranasal live attenuated vaccine, while often produced using egg-based methods historically, is not the non-egg group and isn’t the best example of a non-egg-produced option.